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Journal: Frontiers in Cellular and Infection Microbiology
Article Title: Evaluation of immune sensor responses to a viral small noncoding RNA
doi: 10.3389/fcimb.2024.1459256
Figure Lengend Snippet: mouse TLR3, TLR7 and RIG-I activation by different TMER4 variants. (A) HEK-Blue mTLR3 were tested with different TMER4 variant encoding plasmids and assayed in triplicates. Absorbance was measured at 600-620nm ~16 hours post treatment (n=2). PolyI:C was used for induction at 1µg/mL (B) Similarly, HEK-Blue mTLR7 cell lines were assayed (in triplicates, n=2). EV is empty pUC19 vector. Lipo 3000 is transfection reagent control. CL307 was used the induction control for TLR7. (C) hRIG-I HEK-Lucia cell lines were assayed in triplicates (n=2). 3p-hpRNA is the positive control. Raw luciferase readings are shown in y axis. Statistical analysis was done by GraphPad. There was no statistical difference among tested plasmids. p-Values for empty vector vs. tested plasmids were ranging from 0.2 to 0.99.
Article Snippet: The HEK-Blue mouse TLR (catalog no: hkb-mtlr3, hkb-mtlr4, hkb-mtlr7) and
Techniques: Activation Assay, Variant Assay, Plasmid Preparation, Transfection, Control, Positive Control, Luciferase, Analysis